Today, we did a group experiment on the extraction of DNA from a strawberry. My partner(s) were really helpful and together we finished the experiment successfully.
Summary:
My group and I added alcohol to the mixture, but unfortunately a group member made a mistake and mixed our extract incorrectly. As a result, we had to start over with a fraction of the time left in class, but we managed to finish just in time. Here are the steps we used and I hope you as the reader can follow these steps and perform this experiment yourself:
Procedure (Group Version):
- Get a strawberry, 5 ml of dish soap, 45 ml of water, and 50 milligrams of salt, a test tube, paper towels, plastic seal-able bags and alcohol (depending on how much you make the amount varies). This step is for accumulating you needed items for the experiment.
- Second you put the strawberry in a plastic bag with the soap, the water and the salt.
- Then you crush the strawberry with the mixture of soap, water and salt. The purpose of this step is to break down the strawberry cells to prepare for the being able to see the DNA.
- You then put the paper towel over the test tube (make sure its pushed down a bit so it doesn't leak out). You then pour the smashed strawberry mixture into the test tube. The purpose of this step is to filter out the remainder of the cell but allow the nucleus slip through.
- Next, you pour (roughly) as much alcohol as strawberry mixture into the test tube. They should not mix. The purpose of this step is to break down the nucleus.
- Finally you stir the mixture (only allow your object to descend to the middle instead of all the way down). If you performed all the steps correctly after you finish stirring you should see white strands in the mixture which is DNA.
Procedure (My Version):
- We placed a strawberry in a zip-locked bag
- We poured some buffer of soap detergent into the bag and closed it
- We crushed the strawberries in the bag in order to extract its DNA
- We placed our crushed strawberries into a gauze so that it can be poured into a test tube
- We then took the extract (DNA of synthesis)
We placed the strawberry in zip-lock bag with detergent
We smashed the strawberry mixture and filtered it into a test tube
Our final result of Strawberry DNA (clear solution) in test tube
Notice how the starting point begins with an amino acid group of adenine, thymine, guanine, and ends with thymine, adenine, adenine. Both sequences contain an adenine and a thymine. |
Thanks For Reading!!!
No comments:
Post a Comment